Journal: Emerging microbes & infections

Article Title: Enterovirus-A71 preferentially infects and replicates in human motor neurons, inducing neurodegeneration by ferroptosis.

doi: 10.1080/22221751.2024.2382235

Figure Lengend Snippet: Figure 4. Ferroptosis in human motor neurons after EV-A71 infection. A. Expression levels of FTL and FTH1 after infection in each population. B. Expression levels of FTL and FTH1 after infection in each population with high viral RNA load (High) and low viral RNA load (Low). C. Free irons within the cells were measured by Ferro-Orange. D. Number of cells that were positive for Ferro- Orange in the EV-A71-infected and mock-infected cultures. E. Ferro-Orange intensity in each cell at 6 h after EV-A71 or mock infec tion. n > 1900 cells from 3 cultures, Mann-Whitney Test, ****p < 0.0001. F. Lipid peroxidation measured by BODIPY C11 581/ 591. G. Number of cells displaying oxidized C11 in EV-A71-infected and mock cultures. H. Mean oxidized C11 intensity in each cell at 24 h after EV-A71 or mock infection. n > 2300 cells from 3 cultures, Mann-Whitney Test, ****p < 0.0001.

Article Snippet: The siRNAs used were: SCARB2 (Dharmacon, #012087), FTL (SCBT, sc40577), FTH1 (SCBT, sc-40575).

Techniques: Infection, Expressing, MANN-WHITNEY

Journal: Journal of clinical biochemistry and nutrition

Article Title: Intracellular ferritin heavy chain plays the key role in artesunate-induced ferroptosis in ovarian serous carcinoma cells.

doi: 10.3164/jcbn.21-82

Figure Lengend Snippet: Fig. 3. Effect of knockdown of NCOA4 on ART-induced ferroptosis. (A) Profile of Western blot analysis after transfection of NCOA4 siRNA. (B) Effect of NCOA4 knockdown on ART-induced reduction of cell viability in CaOV3 and SKOV3ip1 cells. (C, D) Effect of NCOA4 knockdown on ART (70 μM)-enhanced lipid peroxidation in CaOV3 cells. C represents profile of BODIPY 581/591 C11. (E, F) Profile of Western blot analysis after trans‐ fection of NCOA4 siRNA and treated by ART in CaOV3 cells. All cells were treated for 24 h. Scrambled siRNA was used as control. Number of samples = 7, *p<0.05.

Article Snippet: NCOA4 siRNA (AM16708, siRNA ID: 107705; Thermo Fisher Scientific) and FTH siRNA (sc-40575; Santa Cruz Biotechnology) were used for gene silencing.

Techniques: Knockdown, Western Blot, Transfection, Control

Journal: Journal of clinical biochemistry and nutrition

Article Title: Intracellular ferritin heavy chain plays the key role in artesunate-induced ferroptosis in ovarian serous carcinoma cells.

doi: 10.3164/jcbn.21-82

Figure Lengend Snippet: Fig. 4. Effect of FTH gene modulation on ART-induced cell death. (A) Profile of FTH level by Western blotting analysis in CaOV3 and SKOV3ip1 cells and FTH silenced in SKOV3ip1 cells by siRNA transfection. (B) Effect of FTH silencing on ART-induced reduction of cell viability in CaOV3 and SKOV3ip1 cells after 24 h of treatment. (C, D) Effect of FTH silencing on ART (70 μM)-enhanced lipid peroxidation in SKOV3ip1 cells after 24 h of treatment. C represents profile of BODIPY 581/591 C11. n = 7. (E, F) Effect of FTH silencing on ART (70 μM)-induced intracellular Fe2+ in SKOV3ip1 cells after 5 h of treatment. E represents profile of Lyso-RhoNox. n = 7. (G) Western blotting analysis showed that FTH was overexpressed in CaOV3 cells through cDNA plasmid transfection. (H) Effect of FTH overexpression on ART-induced reduction of cell viability in CaOV3 after 24 h of treatment. n = 5. (I, J) Effect of FTH overexpression on ART (70 μM)-enhanced lipid peroxidation in CaOV3 cells after 24 h of treatment. I represents profile of BODIPY 581/591 C11. n = 7. (K, L) Effect of FTH overexpression on ART (70 μM)-induced intracellular Fe2+ in CaOV3 cells after 5 h of treat‐ ment. K represents profile of Lyso-RhoNox. n = 7. DMSO was used as vehicle and scrambled siRNA and empty vector (EV) were used as control. FTH cDNA represents FTH overexpression. *p<0.05.

Article Snippet: NCOA4 siRNA (AM16708, siRNA ID: 107705; Thermo Fisher Scientific) and FTH siRNA (sc-40575; Santa Cruz Biotechnology) were used for gene silencing.

Techniques: Western Blot, Transfection, Plasmid Preparation, Over Expression, Control